TECHNOTHROMBIN TGA KIT
Key Features
TECHNOTHROMBIN® TGA measures Thrombin Generation and provides a clinical and research tool for for analysing and monitoring the global function of the haemostatic systemTECHNOTHROMBIN® TGA kit is an assay system for determination of thrombin generation over time in platelet poor or platelet rich plasma (PPP or PRP) after the activation of the clotting cascade by micelles of negatively charged phospholipids containing different amounts of human tissue factor and CaCl2
- A global assay of the haemostasis system
- Highly sensitive test with universal clinical and haemostasis research applications
- Available as a manual test kit, modular reagents or fully automated on Ceveron alpha
- Wide range of standardised TGA trigger reagents for different applications
- Established normal TGA ranges for different patient groups
Product Description
The TECHNOTHROMBIN® TGA kit is an assay system for determination of thrombin generation over time in platelet poor or platelet rich plasma (PPP or PRP) upon activation of the clotting cascade by micelles of negatively charged phospholipids containing different amounts of human tissue factor and CaCl2. The kit can be used to monitor hemophiliacs during inhibitor bypassing therapy, to monitor anticoagulation therapy, to calculate INR values for patients and to determine states of bleeding disorders or thrombophilia as well as the activity of circulating micro particles. This broad range of applications is possible by providing different tissue factor concentrations and by monitoring the whole kinetic of thrombin generation during initiation, amplification and down regulation of thrombin formation.
TECHNOTHROMBIN® TGA is therefore a universal assay kit for analysing and monitoring the function of the haemostatic system.
TECHNOTHROMBIN® TGA is based on monitoring the fluorescence generated by the cleavage of a fluorogenic substrate by thrombin over time upon activation of the coagulation cascade by different concentrations of tissue factor and negatively charged phospholipids in plasma. From the changes in fluorescence over time, the concentration of thrombin (nM) in the sample can be calculated using the respective thrombin calibration curve. The increase in thrombin concentration with time then allows to calculate generation of thrombin in the sample and to plot such thrombin values over time for the whole coagulation process. This then results in the visualisation of the different phases of clot formation.
