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AlloSeq Tx 

Key Features


A new single tube methodology for library preparation that streamlines laboratory workflow and enables full capture of HLA genes, with the opportunity for expandable content targeting additional transplant related genes.

Product Description



AlloSeq Tx - Better Matching. Better Method.


AlloSeq Tx is an innovative NGS HLA typing solution that uses Hybrid Capture Technology to eliminate the inefficiencies and limitations of traditional long-range PCR methods. AlloSeq Tx goes beyond the traditional transplant related loci to consider more transplant associated genes and helps you to identify the best genetic match. When the run is complete, AlloSeq Assign software provides fast import speed and optimised data analysis.


Limitations & Restrictions of Long-Range PCR Benefits of Hybrid Capture
  • Gaps in Current Designs:
    • Not all genes and exons are fully covered.
    • Evidence of beneficial information in UTR regions.
  • Limited Ability to Add Additional Content:
    • e.g., ABO, MIC.
    • Additional PCR reactions required per sample.
  • Data Issues:
    • Allele imbalance and dropouts increase repeat rates.
    • Mis-priming of DNA fragments leads to the generation of hybrid alleles.
  • Workflow Restrictions:
    • Limited scalability; multiple amplification reactions are required per sample.
    • High risk of cross-contamination.
  • Easy Handling:
    • One Sample = One Tube.
    • Early pooling into a single tube.
  • Robust and Reliable Results:
    • More permissive to lower-quality DNA.
    • Captures, rather than amplifies, regions of interest, resulting in less susceptibility to allele dropout.
    • Probe design and spacing further protect against allele dropout and alleviate the need for updating with new allele discovery.
    • Hybrid capture technology is less susceptible to RCP artifacts.
  • Data Quality:
    • Read counts are proportional to copy number.
  • Better Matching:
    • Expandable gene content without impacting the workflow required per sample.
    • Matches for additional MHC markers.

Hybrid capture NGS for robust genetic matching


Alloseq Tx offers a single-tube workflow.


1. Whole Genome Library Generation



2. Hybrid Capture



3. Sequencing



4. Software Analysis



FULL EXONIC AND ENHANCED INTRONIC COVERAGE FOR HLA AND ABO MODULAR CONTENT


AlloSeq Tx moves beyond the traditional transplant related loci to consider more transplant associated genes. Ongoing “Transplantome” discoveries allow addition of new targets to the AlloSeq Tx assay without disrupting workflows.


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